When using micropipettes, where is accuracy generally heightened?

When using micropipettes, accuracy is generally heightened at the top of the range. This is because micropipettes are designed to deliver volumes within a specified range more precisely at the upper limits of their capacity. The mechanics of the pipette and the way liquid is drawn into and expelled from the tip often result in better performance when operating closer to the maximum volume it can handle.

When utilizing a micropipette, it's important to be mindful of the volume range indicated, as operating a pipette at extreme low volumes can lead to inaccuracies due to limitations like subtle variations in the plunger force or volume measurement. Therefore, for the best accuracy and precision, it is recommended to work within the middle to upper range of the pipette's capacity, confirming that the selected volume is a good match for the micropipette's specifications.